Development of single base extension-tags microarray for the detection of food-borne pathogens.
- Author:
Changyong LU
1
;
Chunlei SHI
;
Chunxiu ZHANG
;
Jing CHEN
;
Xianming SHI
Author Information
1. Department of Food Science and Bor Luh Food Safety Center, School ofAgriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
- Publication Type:Journal Article
- MeSH:
Food Contamination;
analysis;
Food Microbiology;
Humans;
Listeria monocytogenes;
isolation & purification;
Oligonucleotide Array Sequence Analysis;
methods;
Salmonella typhimurium;
isolation & purification;
Staphylococcus aureus;
isolation & purification;
Vibrio parahaemolyticus;
isolation & purification
- From:
Chinese Journal of Biotechnology
2009;25(4):554-559
- CountryChina
- Language:Chinese
-
Abstract:
We developed single base extension-tags (SBE-tags) microarray to detect eight common food-borne pathogens, including Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella, Enterobacter sakazaki, Shigella, Escherichia coli O157:H7 and Campylobacter jejuni. With specific PCR primers identified and integrated for eight food-borne pathogens, target sequences were amplified and purified as template DNA of single base extension-tags reaction. The products were hybridized to microarrays and scanned for fluorescence intensity. The experiment showed a specific and simultaneous detection of eight food-borne pathogens. The system limits is 0.1 pg for a genomic DNA and 5x10(2) CFU/mL for Salmonella typhimurium cultures. The single base extension-tags assay can be used to detect food-borne pathogens rapidly and accurately with a high sensitivity, and provide an efficient way for diagnosis and control of disease caused by food-borne pathogens.
