Fusion expression of Escherichia coli heat-labile enterotoxin B subunit gene and foot-and-mouth disease virus type O VP1 gene and immunogenicity analysis.
- Author:
Runcheng LI
1
;
Xinglong YU
;
Xia BAI
;
Weijun XIANG
;
Meng GE
;
Manxiang LI
Author Information
1. College of Animal Medicine, Hunan Agricultural University, Changsha 410128, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
blood;
Bacterial Toxins;
genetics;
immunology;
metabolism;
Capsid Proteins;
genetics;
immunology;
metabolism;
Enterotoxins;
genetics;
immunology;
metabolism;
Escherichia coli;
genetics;
metabolism;
Escherichia coli Proteins;
Female;
Gene Fusion;
genetics;
Mice;
Plasmids;
genetics;
Recombinant Fusion Proteins;
genetics;
immunology;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(4):560-565
- CountryChina
- Language:Chinese
-
Abstract:
LTB gene fragment was amplified by PCR from plasmid pMDTLT, and a recombinant plasmid pETLTBVP1 was constructed by inserting LTB gene fragment into VP1 gene expression plasmid pETVP1 constructed previously. The recombinant plasmids were transformed into E. coli BL21(DE3) and induced to express by IPTG. The recombinant protein existed in the inclusion body and its molecular weight was about 39 kD proved by SDS-PAGE analysis. Western blotting showed that the fusion protein could be reacted with both anti-FMDV and anti-cholera toxin serum demonstrating the immunoactivity of the fusion protein. Strong immune responses can be induced in mice inoculated with the fusion protein intraperitoneally, and the serum antibody level is higher than that of commercial foot-and-mouth disease vaccines.
