Characterization and biocompatibility of human-like collagen-hyaluronic acid scaffold for blood vessel.
- Author:
Xiujuan SUN
1
;
Daidi FAN
;
Chenhui ZHU
;
Xiaoxuan MA
;
Yane LUO
;
Lan CHEN
;
Jiaqing GUO
Author Information
1. Shaanxi Key Laboratory of Degradable Biomaterials, Northwest University, Xi'an 710069, China.
- Publication Type:Journal Article
- MeSH:
Adhesives;
chemistry;
Animals;
Biocompatible Materials;
chemistry;
Biomimetic Materials;
chemistry;
Blood Vessels;
drug effects;
physiology;
Collagen;
chemistry;
Humans;
Hyaluronic Acid;
chemistry;
Iridoid Glycosides;
Iridoids;
chemistry;
Materials Testing;
Mice;
Tissue Engineering;
instrumentation;
methods;
Tissue Scaffolds
- From:
Chinese Journal of Biotechnology
2009;25(4):591-598
- CountryChina
- Language:Chinese
-
Abstract:
Human-like collagen (HLC) was cross-linked with hyaluronic acid by genipin in different ratio. The concentrations of hyaluronic acid in the mixture were 0, 0.01%, 0.05% and 0.1%. The blood vessel tubular grafts were then fabricated by freeze-drying. Microstructure, element composite, mechanical properties, cytotoxicity grade, and biocompatibility of different vascular scaffold groups were studied by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), tensile test, burst pressure experiment, cytotoxicity experiment, endothelial cells planted in blood vessel scaffolds and hypodermic embedding of mice. The results showed that HLC-HA (0.05%) tubular scaffold exhibited interconnected well-distributed and porous structure and porosity of 94.38%; achieved the desirable mechanical property with stress of (1000.8 +/- 7.9) kPa and burst pressure of (1058.6 +/- 8.2) kPa, hypocytotoxicity, favourable cytocompatibility, hisocompatibility and disposition of degradation.
