Construction of anti-H5N1 virus chimeric igA antibody gene and its expression in CHO cells.
- Author:
Baozhong ZHANG
1
;
Xin ZHANG
;
Wanrong CHEN
;
Dabin LIU
;
Sheng WANG
;
Xiaoping AN
;
Duoliang RAN
;
Guangyu ZHAO
;
Yusen ZHOU
;
Yigang TONG
Author Information
1. State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
biosynthesis;
Antibodies, Viral;
genetics;
CHO Cells;
Chimerism;
Cricetinae;
Cricetulus;
Humans;
Immunoglobulin A;
genetics;
immunology;
Influenza A Virus, H5N1 Subtype;
genetics;
immunology;
Mice;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2009;25(5):714-719
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: To express human-mouse chimeric IgA antibody directed against H5N1 virus, an anti-H5N1 chimeric IgA antibody gene was constructed by joining the light and heavy chain variable region genes and the corresponding signal peptide coding sequences of the anti-H5N1 mouse monoclonal antibody H5N1-HA with the coding sequences of the constant region of the human IgA2 heavy chain and Kappa chain respectively. Then the full-length chimeric light and heavy chain expressing plasmids pEF-IGHA9 and pEF-IGK9 were constructed and transfected into the CHO/dhfr cells. The chimeric IgA antibody expression was confirmed by ELISA, SDS-PAGE and Western blotting. The successful expression of this anti-H5N1 chimeric IgA may help to provide a stand for developing passive immunological agents for H5N1 virus infection prophylaxis.
