DNA-PKcs silencing inhibit the DNA repair induced by low dose radiation on human breast epithelial cells.
- Author:
Wei ZOU
1
;
Jian CHE
;
Chongjie WANG
;
Yuying CUI
;
Qinming ZHANG
Author Information
1. College of Life Sciences, Liaoning Normal University, Dalian 116029, China. weizou60@hotmail.com
- Publication Type:Journal Article
- MeSH:
Breast;
cytology;
Cell Line;
DNA Repair;
drug effects;
radiation effects;
DNA-Activated Protein Kinase;
genetics;
Dose-Response Relationship, Radiation;
Epithelial Cells;
metabolism;
radiation effects;
Gene Silencing;
Humans;
Nuclear Proteins;
genetics;
RNA, Small Interfering;
genetics;
Transfection
- From:
Chinese Journal of Biotechnology
2009;25(5):727-732
- CountryChina
- Language:Chinese
-
Abstract:
DNA-PKcs, the catalytic subunit of DNA-dependent protein kinase (DNA-PK), plays an important role in the nonhomologous end-joining (NHEJ) pathway of DNA double-strand breaks (DSBs) repair. To investigate the effects of DNA-PKcs down-regulation on cell growth and sensitization to low dose radiation (LDR), we transfected DNA-PKcs siRNA into human mammary epithelia cells MCF10F, then, detected the proliferation curve of the cells and the expression of protiens in DNA repair pathways. The results showed that DNA-PKcs gene silencing, induced by the transfection of DNA-PKcs siRNA could suppress significantly the cell proliferation and inhibit the expression of the DNA repair proteins, such as Ku80, ATM and P53 after 50 cGy 137Cs gamma-irradiation.The results suggested that DNA-PKcs gene silencing could increase the sensitivity of human breast epithelial cells to the LDR, which might be relative with the decrease of the proteins.
