Establishment of colloidal gold-immunochromatography assay strip for detection of type Asia1 foot-and-mouth disease virus.
- Author:
Tong LIN
1
;
Junjun SHAO
;
Guozheng CONG
;
Junzheng DU
;
Shandian GAO
;
Huiyun CHANG
;
Qingge XIE
Author Information
1. Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chines Academy of Agricultural Science, Lanzhou 730046, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
Chromatography;
methods;
Foot-and-Mouth Disease;
diagnosis;
Foot-and-Mouth Disease Virus;
classification;
immunology;
isolation & purification;
Gold Colloid;
Immunoassay;
methods;
Immunoglobulin G;
immunology;
Reagent Strips;
Sensitivity and Specificity
- From:
Chinese Journal of Biotechnology
2009;25(5):767-772
- CountryChina
- Language:Chinese
-
Abstract:
To establish a sensitive, rapid and simple gold immunochromatography assay (GICA) for detecting Asia1 type of foot-and-mouth disease virus (FMDV) from the field samples. The purified anti-FMDV type Asia1 monoclonal antibody labeled with colloidal gold and the goat anti-Guinea pig IgG were wrapped onto nitrocellulose membrane as the test line (T line) and the control line (C line), respectively. The strip was then further optimized. A total of 87 field samples were detected. The results indicated a correct rate of 98.8% for detecting FMDV Asia1 type. No cross reaction was found with swine vesicular disease (SVD) and FMDV O, A and C type antigen. The sensitivity of the strip can reach to 10(-4) (TCID50 6.25). It had the same results for positive and negative specimens tested in three times. This strip could be stored at 4 degrees C for three months. In this study, the established gold immunochromatographic strip test kit is simple, rapid, sensitive and specific for detecting FMDV type Asial, and is potentially useful for the for pen-side diagnosis.
