Microfluidic cell culture array chip for drug screening assays.
- Author:
Yunhuan ZHENG
1
;
Jianzhang WU
;
Jianbo SHAO
;
Qinghui JIN
;
Jianlong ZHAO
Author Information
1. Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
- Publication Type:Journal Article
- MeSH:
Biosensing Techniques;
instrumentation;
methods;
Cells, Cultured;
Drug Evaluation, Preclinical;
methods;
Endothelial Cells;
cytology;
Hepatocytes;
cytology;
Humans;
Microfluidic Analytical Techniques;
instrumentation;
methods;
Microfluidics;
instrumentation;
methods;
Umbilical Veins;
cytology
- From:
Chinese Journal of Biotechnology
2009;25(5):779-785
- CountryChina
- Language:Chinese
-
Abstract:
We developed a novel microfluidic cell chip, which enabled drug delivery, fluid control and cell co-culture. The device consisted of an array of 6x6 cell culture chambers, a drug gradient generator and fluidic control valves. Micro-dam structures of the chambers were able to trap cells while loading and drug gradient network generated drug gradient of 6 different concentrations. Also we applied hydraulic valves to control the microfluid and simulate the microenvironment of cells. We had investigated the viability of co-culturing cells in the chip and the ability for drug screening. This microfluidic cell chip has the potential in cell-based research of high throughput drug screening.
