Screening and characterization of lipase from a metagenome library of dairy rumen microflora.
- Author:
Shengguo ZHAO
1
;
Jiaqi WANG
;
Kailang LIU
;
Yaxin ZHU
;
Dengpan BU
;
Dan LI
;
Ping YU
Author Information
1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy ofAgriculture Sciences, Beijing 100193, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bacteria;
genetics;
Cattle;
Cloning, Molecular;
Female;
Gene Library;
Lipase;
genetics;
metabolism;
Metagenome;
genetics;
Rumen;
microbiology;
Substrate Specificity;
Temperature
- From:
Chinese Journal of Biotechnology
2009;25(6):869-874
- CountryChina
- Language:Chinese
-
Abstract:
Using lipase segregation agar containing trioleoylglycerol, we obtained 18 lipase positive clones by screening from a metagenome library of dairy rumen microflora containing 15,360 clones. The average insert size of lipase positive clones was about 60 kb. Lipase enzyme activity analysis by p-NPP method indicated that Lipase6, Lipase7 and Lipase8 had higher lipolytic activities to substrates of p-nitrophenyl palmitate (C16), p-nitrophenyl alaurate (C12) and p-nitrophenyl palmitate (C16) respectively. The optimum pH of Lipase 6, Lipase 7 and Lipase 8 were 7.5. The halflife period of Lipase 8 with the value of 15 min in 70 degrees C decreased with the increase of temperature. In conclusion, the lipases screened in this study had different substrates specificity and good thermo stability, which laid a basis for large-scale industrial application.
