Effects of medicinal insect, Catharsius molossus on biosynthesis of polysaccharides from Ganoderma lucidum in submerged culture.
- Author:
Gaoqiang LIU
1
;
Chongyang DING
;
Kechang ZHANG
;
Xiaoling WANG
;
Wenjun HAN
Author Information
1. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China. gaoliuedu@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Cockroaches;
chemistry;
Culture Media;
chemistry;
Fermentation;
Materia Medica;
pharmacology;
Polysaccharides;
biosynthesis;
chemistry;
Reishi;
growth & development;
metabolism
- From:
Chinese Journal of Biotechnology
2009;25(6):914-919
- CountryChina
- Language:Chinese
-
Abstract:
We studied the effects of several medicinal insects on biosynthesis of polysaccharides from Ganoderma lucidum in submerged culture. The results showed that the medicinal insect, Catharsius molossus at 5 g/L significantly promoted the biosynthesis of intracellular polysaccharides (IPS) and extracellular polysaccharides (EPS) of G. lucidum, and compared with control, IPS and EPS yields markedly enhanced from (1.93 +/- 0.09) g/L to (2.41 +/- 0.12) g/L and (520.3 +/- 20.2) mg/L to (608.9 +/- 20.2) mg/L, respectively (P < 0.05). Both IPS and EPS consisted of five kinds of components, and IPS-1 and EPS-1 were the major components of IPS and EPS, respectively. Further separation studies showed that IPS-1 was made up of three single compounds, while EPS-1 was made up of two single compounds. There were no new components in both IPS and EPS obtained from G lucidum in submerged culture by the addition of the insect, C. molossus, suggesting the biosynthetic pathways of the major components of IPS and EPS had not been changed.
