Construction and fermentation of a recombinant Candida glycerinogenes strain with high glycerol production.
- Author:
Ailing LIU
1
;
Zhiming RAO
;
Zheng MA
;
Bin ZHUGE
;
Huiying FANG
;
Jian ZHUGE
Author Information
1. The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
- Publication Type:Journal Article
- MeSH:
Agrobacterium tumefaciens;
enzymology;
genetics;
Candida;
genetics;
metabolism;
Electroporation;
Fermentation;
Glycerol;
analysis;
metabolism;
Glycerolphosphate Dehydrogenase;
genetics;
Recombination, Genetic;
Transformation, Genetic
- From:
Chinese Journal of Biotechnology
2009;25(6):946-952
- CountryChina
- Language:Chinese
-
Abstract:
Candida glycerinogenes WL2002-5 (C.g) is an important industrial strain for glycerol production. To further improve glycerol production, we reconstructed a binary vector pCAM3300-zeocin-CgGPD1, introduced it to Agrobacterium tumefaciens LBA4404 by electroporation, and then transformed the T-DNA harboring the CgGPD1 to Candida glycerinogenes by Agrobacterium tumefaciens-mediated transformation (ATMT). After 96 h fermentation with glucose as the substrate, we screened a transformant named C.g-G8 with high glycerol production. Compared with the wild strain, the glucose consumption rate of C.g-G8 and the glycerol production were 12.97% and 18.06% higher, respectively. During the fermentation, the activity of glycerol-3-phosphate dehydrogenase of C.g-G8 was 27.55% higher than that of the wild strain. The recombinant Candida glycerinogenes with high glycerol production was successful constructed by ATMT method.
