Construction of an infectious cDNA clone derived from foot-and-mouth disease virus O/QYYS/s/06.
- Author:
Shousheng LU
1
;
Qizu ZHAO
;
Xiangtao LIU
;
Yanwei SUN
;
Tao REN
;
Guihong ZHANG
;
Wenbao QI
;
Yunfeng ZHA
;
Lingchen KONG
;
Han ZHANG
;
Huiying FAN
;
Ming LIAO
Author Information
1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Cloning, Molecular;
DNA, Complementary;
genetics;
DNA, Viral;
biosynthesis;
genetics;
Foot-and-Mouth Disease;
virology;
Foot-and-Mouth Disease Virus;
classification;
genetics;
pathogenicity;
Mice;
Transcription, Genetic;
Transfection
- From:
Chinese Journal of Biotechnology
2009;25(7):982-986
- CountryChina
- Language:Chinese
-
Abstract:
After sequencing, we amplified and cloned foot-and-mouth disease virus (FMDV) O/QYYS/s/06 whole genome by three fragments. These three fragments were cloned into vector P43 one by one to construct recombinant plasmid P43C, which carried the full-length cDNA of FMDV O/QYYS/s/06. Then, plasmid P43C and plasmid T7 expressing T7 RNA polymerase were co-transfected into BHK-21 cells. After 48 h, we harvested the culture broth from transfected BHK-21 cells and inoculated into 2-3 day-old sucking mice. After four generation passage, the virus harvested from sucking mice was confirmed to be type O FMDV by the indirect hemagglutination test, sucking mice's neutralization test and sequencing. The results showed that we have successfully constructed the full-length cDNA clone of FMDV O/QYYS/s/06 strain.
