Determination of 2, 4-toluenediamine in urine by gas chromatography.
- Author:
Wan-chao ZHANG
1
;
Bao-feng LIU
;
Hui LIU
;
Rong-ming MIAO
2
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Gas; methods; Humans; Occupational Exposure; analysis; Phenylenediamines; urine
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(12):939-940
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determining the content of 2,4-toluenediamine, a urinary metabolite of toluene diisocyanate, by gas chromatography.
METHODSUrine samples were collected, and acidification, extraction, derivatization, separation with a capillary column, and detection with an electron capture detector were performed. The target compound was qualified by retention time and quantified by peak area.
RESULTSThe concentration of 2, 4-toluenediamine showed a linear relationship with peak area within 0.0∼40 ng/ml, with a correlation coefficient 0.9995; the limit of detection was 0.44 ng/ml, the lower limit of quantification was 1.47 ng/ml, the relative standard deviation was 1.85%∼4.05%; the recovery rate was 97.98%∼99.28%.
CONCLUSIONThe method has the advantages of high sensitivity and high accuracy and can be used for determination of 2, 4-toluenediamine in urine.