FISH analysis of meiotic segregation results of the spermatozoa from male pericentric inversion carriers.
- Author:
Cheng-Shuang PAN
1
;
Xiu-Fang QIU
;
Xi-Xi HUANG
;
Zhi-Liang WENG
;
Xue-Feng HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Chromosome Inversion; genetics; Chromosomes, Human, Pair 9; genetics; Heterozygote; Humans; In Situ Hybridization, Fluorescence; methods; Infertility, Male; genetics; Male; Meiosis; Spermatozoa
- From: National Journal of Andrology 2012;18(4):344-348
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the meiotic segregation results of the spermatozoa from male pericentric inversion carriers by fluorescence in-situ hybridization (FISH).
METHODSUsing chemical depolymerization and multicolor FISH, we analyzed the meiotic segregation results of the spermatozoa from 4 male pericentric inversion carriers.
RESULTSOf the 4 males studied, 46,XY,inv(9) (p11q12) was found in 2, 46,XY,inv(9) (p11q13) in 1 and 46,XY,inv(6) (p22q24) in the other; the lengths of the inverted segments represented 16.0, 16.0, 21.0 and 76.0% of the size of the whole chromosome involved; and the frequencies of recombinant sperm were 0.2, 0.4, 0.3 and 43.9%, del(p)/dup(q) accounting for 22.4% and del(q)/dup(p) 21.5%, respectively.
CONCLUSIONMales with pericentric inversion may produce spermatozoa with recombinant chromosomes and the rate of recombination varies principally according to the size proportion to the whole chromosome involved. The results of FISH analysis of chromosomal unbalanced spermatozoa can provide accurate personalized information on the genetic risk of fertility.
