Breakpoints located by sequence tagged sites of AZFc microdeletion in Chinese Han population.
- Author:
Qing WU
1
;
Lei-Guang WANG
;
Bin WU
;
Yi QIU
;
Yan XU
;
Miao LIU
;
Peng WANG
;
Yao YUAN
;
Hui-Juan SHI
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; Azoospermia; genetics; Chromosome Deletion; Chromosomes, Human, Y; Humans; Infertility, Male; genetics; Male; Sequence Tagged Sites
- From: National Journal of Andrology 2012;18(5):387-390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the breakpoints of the azoospermia factor c (AZFc) microdeletion in Chinese Han population.
METHODSWe detected 9 sequence tagged sites (sY84, sY86, sY127, sY134, sY152, sY145, sY255, sY254 and sY157) to confirm AZFc microdeletions in the Y chromosome for patients with severe oligozoospermia or non-obstructive azoospermia by multiplex polymerase reaction. To locate the breakpoints of AZFc microdeletions, we analyzed 192 patients with sY255, sY254 and sY157 dele- ted by detecting sY1191, sY1197, sY1054, sY1125 and sY1206, respectively.
RESULTSFive breaking patterns were found in the 192 patients with sY255, sY254 and sY157 deleted, among which the common ones were sY1197(+), sY1191(-), sY1054(-), sY1206(-) and sY1125(+), which accounted fro 54.17% (104/192), sY1197(+), sY1191(+), sY1206(-), sY1054(-) and sY1125(+), which constituted 28.12% (54/192), sY1197(+), sY1191(-), sY1206(-), sY1054(+) and sY1125 (+), which made up 14.58% (28/192). The proximal breakpoint located between sY1197 and sY1191 was 70.83% of AZFc microdeletions, and the distant breakpoint located between sY1054 and sY1125 was 82.29%.
CONCLUSIONThere are 5 breaking patterns of AZFc microdeletions in Chinese Han population, the proximal and distant breakpoints mostly located at the replicons b2 and b4, respectively.
