Expression of TEKT4 protein decreases in the ejaculated spermatozoa of idiopathic asthenozoospermic men.
- Author:
Wen-Bin WU
1
;
Yu-Shan LI
;
Xiao-Fei JI
;
Quan-Xian WANG
;
Xue-Min GAO
;
Xian-Feng YANG
;
Zhou-Hui PAN
;
Xiao-Xia FENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Asthenozoospermia; metabolism; Blotting, Western; Case-Control Studies; Cytoskeletal Proteins; metabolism; Humans; Male; RNA, Messenger; genetics; Sperm Motility; Spermatozoa; metabolism
- From: National Journal of Andrology 2012;18(6):514-517
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of the TEKT4 protein in the pathogenesis of idiopathic asthenozoospermia.
METHODSWe separated and purified the ejaculated sperm from idiopathic asthenozoospermia patients and normozoospermic men by Percoll discontinuous density gradients, and detected the distribution and the expressions of TEKT4 mRNA and TEKT4 protein by RT-PCR and Western blot.
RESULTSRT-PCR revealed that the expression of TEKT4 mRNA was significantly lower in the sperm of the idiopathic asthenozoospermia patients than in those of the normozoospermic men (0.59 +/- 0.13 vs 0.75 +/- 0.15, t = 4.325, P < 0.05), and Western blot confirmed the results of RT-PCR (0.48 +/- 0.14 vs 0.69 +/- 0.13, t = 5.939, P < 0.05).
CONCLUSIONThe expression of TEKT4 is significantly decreased in the ejaculated sperm of idiopathic asthenozoospermia patients, which might be one of the causes of idiopathic asthenozoospermia.
