Effects of PPAR-gamma on the proliferation and glycolysis metabolism of prostate cancer cells.
- Author:
Xiang-Jian ZENG
1
;
Xue-Cheng BI
;
Qi-Shan DAI
;
Zhao-Dong HAN
;
Wei-De ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Genetic Vectors; Glucose Transporter Type 1; metabolism; Glycolysis; Humans; Male; PPAR gamma; genetics; metabolism; Prostatic Neoplasms; metabolism; pathology; Proto-Oncogene Proteins c-myc; metabolism; RNA Interference; RNA, Small Interfering; Transfection
- From: National Journal of Andrology 2012;18(8):692-696
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of the expression of the PPAR-gamma gene on the proliferation and glycolysis metabolism of prostate cancer cells.
METHODSUsing RNAi, we constructed lowly--expressed shRNA-PPARgamma adenoviruses and transfected them to PC3 prostate cancer cells, with blank vectors as controls. Then we detected the proliferation and apoptosis of the cells, glycolysis metabolism related genes and lactate accumulation by CCK-8 kit, and compared the results between the two groups.
RESULTSCompared with the control group, the PPAR-gamma gene expression was obviously inhibited by RNAi in the PC3 cells, and its protein expression was reduced to (26.00 +/- 4.06)%. The proliferation inhibition rate was (39.5 +/- 4.92)% on the 2nd day, and the apoptosis rate was as high as (21.03 +/- 3.08)%. The glycolysis metabolism related gene products (Myc and Glut-1) were significantly decreased, and the lactate concentration was reduced to 69.71% of that of the controls on the 4th day. There were statistically significant differences in the above findings as compared with the control group (P < 0.01).
CONCLUSIONPPAR-gamma gene knockdown is expected to be a new way to treat prostate cancer.
