Nuclear matrix proteins differentially expressed in human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line.
- Author:
Tie-Cheng SUN
1
;
Ling XIN
;
Li-Ming SONG
;
Yue ZHOU
;
Li-Feng NING
;
Li-Yuan HAN
;
Ying GUO
;
He-Ming YU
;
Hui-Ping WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Cell Line, Tumor; Electrophoresis, Gel, Two-Dimensional; Humans; Male; Nuclear Matrix-Associated Proteins; metabolism; Prostatic Hyperplasia; metabolism; Prostatic Neoplasms; metabolism; Proteome; analysis; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: National Journal of Andrology 2012;18(7):583-589
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the expression of nuclear matrix proteins (NMPs) in benign prostatic hyperplasia (BPH) epithelial cell line BPH-1 versus those in androgen-dependent human prostate cancer cell line LNCap and androgen-independent prostate cancer cell line PC-3.
METHODSWe isolated NMPs from the BPH-1, LNCap and PC-3 cell lines by 2-dimensional electrophoresis (2-DE), analyzed the differentially expressed proteins by matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF-MS), and identified them by peptide mass fingerprint and database searching.
RESULTSWe successfully obtained well-resolved reproducible 2-DE patterns of NMPs in human prostate cancer cell lines, identified 12 differentially expressed NMPs including enzymes, regulatory proteins, RNA-binding protein and various other factors, 3 up-regulated and 9 down-regulated in prostate cancer cell lines.
CONCLUSIONThere are obvious differences in the expressions of NMPs between human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line.
