Effect of Peimine on ERCC1 mRNA and LRP Expressions of A549/DDP Multidrug Resistance Cell Line.
- Author:
Xiao-yong TANG
;
Ying-xue TANG
;
Peng XU
;
Hai-yan ZHOU
;
Li HAN
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cevanes; pharmacology; Cisplatin; DNA-Binding Proteins; genetics; Down-Regulation; Drug Resistance, Multiple; Drug Resistance, Neoplasm; drug effects; Endonucleases; genetics; Humans; Low Density Lipoprotein Receptor-Related Protein-1; genetics; Lung Neoplasms; RNA, Messenger; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1490-1494
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of peimine on excision repair cross-complementation 1 (ERCC1) mRNA and lung resistant protein (LRP) expressions in A549/cisplatin (DDP) multidrug resistance (MDR) cell line.
METHODSLung cancer A549/DDP cells were cultured in vitro.Cells at logarithmic growth phase were divided into 4 groups, i.e., the blank control group, the DDP group, the ligustrazine group (DDP+ligustrazine), the peimine group (DDP + peimine). After 48-h drug action, ERCC1 mRNA expression was detected by RT-PCR and LRP expression detected by cell immunofluorescence.
RESULTSThere was no statistical difference in expression levels of ERCC1 mRNA and LRP between the DDP group and the blank control group (P > 0.05). Compared with the DDP group, expression levels of ERCC1 mRNA and LRP obviously decreased in the ligustrazine group and the peimine group (P < 0.05). They were obviously lower in the peimine group than in the ligustrazine group (P < 0.05).
CONCLUSIONSPeimine could reverse MDR of A549/DDP cell line. Its mechanism might be associated with down-regulating ERCC1 mRNA and LRP expression levels.