Pharmacokinetic Effect of Aikeqing Granule by Different Medication Ways on Zidovudine in HAART of Rats.
- Author:
Zhen-zhen LU
;
Qi-jian SU
;
Jia-bao MA
;
Dan-hui TANG
;
Ce SONG
;
Lin-chun FU
- Publication Type:Journal Article
- MeSH: Animals; Antiretroviral Therapy, Highly Active; Benzoxazines; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; pharmacokinetics; pharmacology; Mass Spectrometry; Rats; Zidovudine; pharmacokinetics; pharmacology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1501-1504
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study pharmacokinetic effect of Aikeqing Granule (AG) by different medication ways on zidovudine (AZT) in highly active antiretroviral therapy ( HAART) of rats.
METHODSTotally 36 rats were administered with corresponding medications by gastrogavage, group I [HAART: AZT 31.5 mg/kg +3TC 31.5 mg/kg + Efavirenz (EFV) 63.0 mg/kg], group II (HAART+AG525 mg/kg), group III (HAART and AG 525 mg/kg after a 2-h interval). Drug concentrations of AZT were determined by high performance liquid chromatography-mass spectroscopy (HPLC-MS) before HAART, and at 0.5, 1, 2, 3, 4, 6, 8, 10, 12 h after HAART, respectively. Pharmacokinetic parameters [such as t1/2, Tmax, Cmax, AUCo-t, plasma clearance rate (CL)] were calculated by DAS2.0 Software.
RESULTSThe-equation of linear regression of AZT was good, with the precision, coefficient of recovery, and stability definitely confirmed. AUC in group II and III was larger than that of group I. There was no statistical difference in t1/2, Tmax, Cmax, AUC0-12 h, or AUC0-∞ among groups (P > 0.05).
CONCLUSIONAG combined HAART could enhance the Cmax of AZT.