Xianglian External Lotion Restored the Sensitivity of Drug-resistant Candida albicans Strains to Fluconazole: a Transcriptomics Study.
- Author:
Ping WANG
;
Zhi-qi FAN
;
Rui-qiang FAN
- Publication Type:Journal Article
- MeSH: Antifungal Agents; pharmacology; therapeutic use; Candida; Candida albicans; drug effects; genetics; Candidiasis; drug therapy; Drug Resistance, Fungal; drug effects; genetics; Drugs, Chinese Herbal; pharmacology; therapeutic use; Fluconazole; pharmacology; Microbial Sensitivity Tests
- From: Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1505-1509
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo perform a transcriptomics study in differential genes after Xianglian External Lotion (XEL) induced the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole.
METHODSBroth microdilution antifungal susceptibility test was used to detect minimal inhibitory concentration (MIC) of drug-resistant Candida albicans strains induced by XEL. Transcriptome sequencing (RNA-Seq) was used to determine and compare the transcription of primary drug-resistant Candida aIbicans strains and sensitive strains induced by XEL. High expressed genes and signaling pathways strains were analyzed by gene ontology (GO) method.
RESULTSXEL could induce drug-resistant strains of the 6th generations to recover sensitivity. Transcriptome sequencing showed that, as compared with primary drug-resistant strains, there were 165 genes with up-regulated RPKM index and 144 genes with down-regulated RPKM index after XEL induction. GO analyses found that all genes were mainly classified as GO:0015903 (fluconazole transport).
CONCLUSIONSXEL could induce the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole. By analyzing transcriptomes, authors speculated that XEL could recover strain sensitivity to fluconazole by opening fluconazole transport pathway.