Expression of human LNalpha4LG4-5 in Pichia yeast.
- Author:
Ji-Qin LIAN
1
;
Xu-Fang DAI
;
Xiao-Hui LI
;
Feng-Tian HE
Author Information
1. Faculty of Basic Medical Science, Third Military Medical University, Chongqing 400038, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Genetic Vectors;
Humans;
Laminin;
biosynthesis;
genetics;
Pichia;
genetics;
metabolism;
Plasmids;
genetics;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
isolation & purification
- From:
Chinese Journal of Biotechnology
2006;22(2):226-230
- CountryChina
- Language:Chinese
-
Abstract:
To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
