The application of co-culture system on the in vitro development of bovine somatic nuclear transferred embryos.
- Author:
Yue-Hong WU
1
;
Zhi-Xing AN
;
Yong ZHANG
;
Xiang-Chen LI
;
Yu-Long HE
;
Feng-Jun LIU
Author Information
1. Bioengineering Institute, Northwest Agricultural & Forstry University, Yangling 712100, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Cellular Reprogramming;
Cloning, Organism;
methods;
Coculture Techniques;
Culture Media;
Embryo, Mammalian;
cytology;
drug effects;
Embryonic Development;
Epithelial Cells;
cytology;
drug effects;
Fallopian Tubes;
cytology;
Female;
Granulosa Cells;
cytology;
drug effects;
Nuclear Transfer Techniques
- From:
Chinese Journal of Biotechnology
2006;22(2):306-310
- CountryChina
- Language:Chinese
-
Abstract:
To establish a co-culture system of nuclear transferred embryos in bovine, effects of co-culture cell types, passages and cryopreservation as well as addition of BFF or FBS were investigated. The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P < 0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell. Passages of co-culture cells significantly affected the blastocyst rate and embryo cell number (P < 0.05), and cryopreservation decreased the blastocyst rate and embryo cell number remarkably. Supplemention of BFF increased blastocyste rate significantly (P < 0.05). In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10% BFF in SOFaa could improve development of nuclear transferred bovine embryo in vitro.
