The effect of oncolytic adenovirus on human umbilical vein endothelial cell.
- Author:
Zhen-Min YE
1
;
Xiao-Hua WANG
;
Jiang ZHONG
;
Jing-Cheng MIAO
;
Wei-Hua SHENG
;
Yu-Feng XIE
;
Jin-Zhi WANG
;
Ji-Cheng YANG
Author Information
1. Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
physiology;
Adenovirus E1A Proteins;
biosynthesis;
genetics;
Cell Proliferation;
Cells, Cultured;
Endothelial Cells;
cytology;
metabolism;
Humans;
Oncolytic Virotherapy;
Oncolytic Viruses;
genetics;
physiology;
Promoter Regions, Genetic;
Umbilical Veins;
cytology;
metabolism
- From:
Chinese Journal of Biotechnology
2006;22(3):397-402
- CountryChina
- Language:Chinese
-
Abstract:
The E1A gene was obtained by PCR with QBI-293A cell genome DNA as template. After enzyme digestion, the E1A gene was ligated to transfer vector pAdTrack-CMV. The positive clone pAdTrack-CMV-E1A were lineared by PmeI and co-transformed with pAdEasy-1 in BJ5183 E. coli. The recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-E1A were digested by PacI and transfected into QBI-293A cells with liposomes. The oncolytic recombinant adenovirus Ad-E1A was obtained after 7 days. The results showed that this oncolytic adenovirus Ad-E1A can replicate in ECV304 cells and inhibit growth of ECV304 cell. In addition, it also decreased the secretion of VEGF and expression of NF-kappaB of ECV304 cells, indicating that Ad-E1A have potential of inhibition of tumor metastasis.
