Study of fusion protein and attachment glycoprotein of Nipah virus expressed in recombinant baculovirus.
- Author:
Xi-Jun WANG
1
;
Sen HU
;
Jin-Ying GE
;
Qing-Hua WANG
;
Li-Ting QIN
;
Zhi-Gao BU
Author Information
1. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, Viral;
immunology;
Baculoviridae;
genetics;
metabolism;
Mice;
Mice, Inbred BALB C;
Nipah Virus;
chemistry;
genetics;
Rabbits;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
immunology;
Recombination, Genetic;
Viral Envelope Proteins;
biosynthesis;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2006;22(3):418-424
- CountryChina
- Language:Chinese
-
Abstract:
In this study, Recombinant baculoviruses rBac-NF and rBac-NG were generated for expressing F and G proteins Nipah virus (NiV) . The expression of recommbinant G (rNG) and F (rNF) protein in rBac-NF and rBac-NG infected cells were confirmed by western-blot. Both rNG and rNF showed sensitive and specific antigenic reaction to rabbit serum anti-Nipah virus in indirect immunofluorescence detection and indirect ELISA. Immunization with rBac-NF and rBac-NG infected insect cells elicited G ad F protein specific antibody responses in mice. Furthermore, the G ad F specific antibodies could neutralize the infectivity of the VSVdeltaG* F/G, the NiV F and G envelope glycoproteins psudotyped recombinant Vesicular Stomatitis Virus expressing green fluorescence protein. The results demonstrated F and G protein expressed by the recombinant baculoviruses could be safe economic diagnostic antigens for the surveillance and monitoring of NiV and promising subunit vaccines for the prevention of NiV.
