A new purification methods of small DNAs--the purification methods with silica wool.
- Author:
Jing ZHANG
1
;
Tian-Yi YING
;
Chuan GAO
;
Yun-Yang SONG
;
Wei-Tao HAN
;
Hui-Fang WANG
Author Information
1. Beijing Research Institute of Chemical Defense, Beijing 102205, China.
- Publication Type:Journal Article
- MeSH:
DNA;
genetics;
isolation & purification;
Electrophoresis, Agar Gel;
methods;
Silicon Dioxide;
chemistry
- From:
Chinese Journal of Biotechnology
2006;22(3):504-507
- CountryChina
- Language:Chinese
-
Abstract:
The principal purpose of this study is to set up efficient purification techniques of small DNAs which are suitable for isolation of from tens to three hundred bases of genes. On the bases of the technique, purification methods for big DNA fragments are established. In the experiment, the DNA bands were cut after agarose gel electrophoresis and put into 0.5 mL of tubes with silica wool, glass wood, absorbent cotton and cotton at the bottom. And then 10 000 r/min for 2 min, the liquid was collected. The results indicated that silica wool was the best of the materials. The recovery rate for DNAs below 200bp was over 90%, 85% to approximately 90% for 300bp. And the technique can be applied to purify bigger DNA fragments. The kits for DNA purification hardly recovered DNA below 150bp. The recovery rate for 150bp of DNA was 5%, 60% even for 300bp. The efficiencies of enzymic digestion and enzymic connection for the DNAs purified by the technique were the same as those for the DNAs isolated by the kits. So, the technique is obviously superior to kit purification methods.
