Fusion tags technology and their applications.
- Author:
Yong-Jin LI
1
;
Yuan-Yuan CHEN
;
Li-Jun BI
Author Information
1. Protein Research Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
- Publication Type:Journal Article
- MeSH:
Recombinant Proteins;
chemistry;
isolation & purification;
Solubility
- From:Chinese Journal of Biotechnology
2006;22(4):523-527
- CountryChina
- Language:Chinese
-
Abstract:
Fusion tags are originally developed to facilitate the purification of recombinant protein from crude extracts. In recent years, the discovery of different tags and the development of fusion strategy make the function of fusion tags diversified. However, there was no a cure-all fusion tag for different applications. We here give an overview of fusion tag technology and the different applications of fusion tags, including the purification, detection and oriented immobilization of recombinant protein, the visualization of bioevent in vivo, the enhancement of the yield of protein, the improvement of the solubility and stability of the expressed protein.
