A novel binary vector to get marker-free transgenic plant.
- Author:
Xia LI
1
;
Hai-Bo WENG
;
Shao-Yin HAN
;
Yu XI
;
Ke-Lan YONG
Author Information
1. College of Life Sciences of Shanghai University, Shanghai 200436, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Genetic Vectors;
genetics;
Integrases;
metabolism;
Molecular Sequence Data;
Plants, Genetically Modified;
genetics;
Recombination, Genetic;
Tobacco;
genetics
- From:
Chinese Journal of Biotechnology
2006;22(4):550-554
- CountryChina
- Language:Chinese
-
Abstract:
A novel practical binary vector to get marker-free transgenic plant was constructed. The estrogen-inducible Cre/loxP DNA recombination system was adopted in this system. All non-target genes located between two identical orientation loxP sites could be excised from the transgenic genome by the Cre expression. In order to analyze this system, the target gene, GUS expression box (CaMV35s: :GUS), was inserted in the MCS outside the region franked by two loxP sites. Then it was introduced into the tobaccos. Results showed that the high-efficiency DNA recombination had take place and the target gene was working order after DNA excitation.
