Effect of phosphate on growth and polysaccharide production by suspension cultures of protocorm-like bodies of Dendrobium huoshanense.
- Author:
Shao-Tong JIANG
1
;
Ming WEI
;
Jian-Ping LUO
Author Information
1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. jiangst@hfut.edu.cn
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
drug effects;
Dendrobium;
growth & development;
metabolism;
Phosphates;
metabolism;
pharmacology;
Polysaccharides;
biosynthesis;
Suspensions
- From:
Chinese Journal of Biotechnology
2006;22(4):613-618
- CountryChina
- Language:Chinese
-
Abstract:
The effect of inorganic phosphate on cell growth, accumulation of polysaccharides together with nutrient utilization was investigated in suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Thirty-day-old cells were transferred into liquid medium with the inoculum density of 100 g/L cells. The results indicate that the optimal concentration of phosphate in medium for cell growth of protocorm-like bodies of Dendrobium huoshanense was 2.5 mmol/L and biomass was 496.5 g/L (fresh weight). Phosphate was a limited factor for cell growth and there was relationship between the levels of intracellular phosphate and cell growth. 2.5 mmol/L of medium phosphate was beneficial to the absorption of carbohydrate and nitrate source. 0.312 mmol/L of medium phosphate was better for accumulation of polysaccharides and production of polysaccharides was 2.22 g/L at 36 d.
