Isolation and identification of lipopeptides produced by Bacillus subtilis fmbJ.
- Author:
Xiao-Mei BIE
1
;
Feng-Xia LÜ
;
Zhao-Xin LU
;
Xian-Qing HUANG
;
Juan SHEN
Author Information
1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
- Publication Type:Journal Article
- MeSH:
Anti-Infective Agents;
chemistry;
isolation & purification;
Bacillus subtilis;
metabolism;
Chromatography, High Pressure Liquid;
Chromatography, Thin Layer;
Lipopeptides;
chemistry;
isolation & purification;
Spectrometry, Mass, Electrospray Ionization
- From:Chinese Journal of Biotechnology
2006;22(4):644-649
- CountryChina
- Language:Chinese
-
Abstract:
Isolation and idenfication of lipopeptides from Bacillus subtilis fmbJ was carried out in this paper. With HPLC method, it was determined that the antimicrobial substance was composed of many components, and one of them had the similar retention time similar to surfactin. In addition, the antimicrobial substance was proved to include the closed cycle peptide bind by TLC, and one of them had the migrating rate similar to surfactin. Furthermore, ESI-MS analysis showed that the antimicrobial substance contained five homologues of fengycin, such as m/z1449.9, m/zl1463.8, m/zl1477.8, m/z1491.9 and m/z1505.9, and three homologues of surfactin, such as m/z1008.8, m/z1022.8 and m/z1036.8.
