Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides.
- Author:
Yong-Hong LI
1
;
Bo LIU
;
Zong-Bao ZHAO
;
Feng-Wu BAI
Author Information
1. Department of Bioscience and Bioengineering, Dalian University of Technology, Dalian 116023, China.
- Publication Type:Journal Article
- MeSH:
Basidiomycota;
growth & development;
metabolism;
Copper;
pharmacology;
Culture Media;
Fermentation;
Hydrogen-Ion Concentration;
Lipids;
biosynthesis;
Magnesium Sulfate;
pharmacology;
Zinc;
pharmacology
- From:
Chinese Journal of Biotechnology
2006;22(4):650-656
- CountryChina
- Language:Chinese
-
Abstract:
Culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides were optimized with single factor and uniform design experiment. The best medium recipe was found with 70 g/L glucose, 0.1 g/L (NH4)2SO4, 0.75 g/L yeast extract, 1.5 g/L MgSO4. 7H2O, 0.4g/L KH2PO4, sterilized at 121 degrees C for 15 min, and then supplemented with ZnSO4 1.91 x 10(-6) mmol/L, CaCl2 1.50 mmol/L, MnCl2 1.22 x 10(-4) mmol/L and CuSO4 1.00 x 10(-4) mmol/L. The optimal fermentation conditions were as follows: 50 mL of medium (pH 6.0) in 250 mL Erlenmeyer flask with 10% inoculum (28h) under orbital shaking at 200 r/min for 120h at 30 degrees C. Under these conditions, yeast biomass accumulated lipids up to 76.1%.
