Screen of media for insect cells (HzAm1) growth and its adaptation to low serum culture.
- Author:
You-Hong ZHANG
1
;
Hua-Lin WANG
;
Xiong-Wei ZHU
;
Qin QIN
;
Yan CHEN
;
Zhong LÜ
;
Jie MA
;
Jing ZHANG
Author Information
1. Hubei Key Laboratory of Novel Chemical Reactor & Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China. youhong64@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Adaptation, Physiological;
Animals;
Cell Proliferation;
Culture Media;
Culture Media, Serum-Free;
Lepidoptera;
cytology
- From:
Chinese Journal of Biotechnology
2006;22(4):686-688
- CountryChina
- Language:Chinese
-
Abstract:
Insect cell-baculovirus system is a useful tool for both insecticidal virus production and the expression of medically useful foreign genes. Serum-free culture or low serum culture for insect cells is essential and significant. Media for the growth of insect cells HzAm1 from three kinds of commercial media TC-100,GRACE and IPL-41 with 10% serum were investigated and screened. The result shows that medium TC-100 is the most suitable medium for the growth of cells HzAm1. Adaptation of insect cells HzAm1 to cultures in medium TC-100 with serum reduction from 10% to 1% was carried out as cultures were supplemented with lactalbumin hydrolysate and yeastolate etc. Growth of insect cells HzAml in medium TC-100 with serum 1%, lactalbumin hydrolysate and yeastolate was well.
