Genotype distribution of enterovirus group C in Shandong Province, China.
- Author:
Yan LI
1
;
Yao LIU
;
Hai-yan WANG
;
Ze-xin TAO
;
Li-zhi SONG
;
Gui-fang LIU
;
Lei FENG
;
Yi FENG
;
Xiao-juan LIN
;
He YANG
;
Qing-ying FAN
;
Ai-qiang XU
Author Information
1. Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan 250014, China. liyan7516@163.com
- Publication Type:Journal Article
- MeSH:
Cell Line;
China;
Enterovirus C, Human;
classification;
genetics;
Genotype;
Humans;
Molecular Sequence Data;
Phylogeny;
Reverse Transcriptase Polymerase Chain Reaction
- From:Chinese Journal of Virology
2010;26(5):363-367
- CountryChina
- Language:Chinese
-
Abstract:
Human Enterovirus C group (HEV-C) includes 17 serotypes, which can not be serotype-identified by neutralization test using antiserum pool for NPEV. In order to elucidate the genotypes and molecular evolution of HEV-C in Shandong Province, We selected the strains isolated from AFP cases between 1994-2009 to perform reverse transcriptase-polymerase chain reactions (RT-PCR) by the primers specific for entire VP1 coding gene of HEV-C and sequencing. The phylogenetic tree was then constructed among these VP1 nucleotide sequences and other prototype strains. Totally 12 Shandong local strains were obtained and separated into 4 genotypes, CVA20, CVA21,CVA24 and EV 96. The homologous comparison and phylogenetic analysis showed Shandong strains were distinct from prototype strains in each genotype. This report showed that different genotype HEV-C strains spread widely in Shandong Province.
