Study on the inhibitory effect of RNA interference on replication of dengue virus.
- Author:
Jin-ya YUE
1
;
Xin-wei WU
;
Ye-jian WU
;
Xiang-zhong LI
;
Li-yun JIANG
;
Qiao-yan LI
;
Lei LI
;
Xia YANG
Author Information
1. Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Dengue Virus;
genetics;
physiology;
RNA Interference;
physiology;
RNA, Small Interfering;
genetics;
RNA, Viral;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Virus Replication;
genetics;
physiology
- From:
Chinese Journal of Virology
2010;26(5):373-378
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the inhibitory effect of RNA interference (RNAi) on dengue virus I (DENV-1) replication. Small interfering RNA (siRNA) against the PreM gene of dengue virus was synthesized and transfected into C6/36 cells with liposome, which was then attacked by DENV-1 virus. The antiviral effect of siRNA was evaluated by cytopathic effect (CPE), the cell survival rate measured by MTT, and virus RNA quantified by real-time RT-PCR. The results showed that after 7 days post infection of dengue virus, the transfected C6/36 cells showed less CPE. The cell survival rate of the transfected C6/36 cells increased by 2.26 fold, and the amount of virus RNA in the transfected cells was reduced by about 97.54% as well. These findings indicated that the siRNA could effectively inhibit dengue virus RNA replication, and protect C6/36 cells from viral attack, indicating its potential role in prevention and treatment of dengue fever.
