Detection of molecular markers of amantadine resistance in avian influenza viruses by pyrosequencing.
- Author:
Hua-lei LIU
1
;
Yan LV
;
Dong-xia ZHENG
;
Yun-ling ZHAO
;
Cheng-ying SUN
;
Wei ZHANG
;
Jin-ming LI
;
Zhi-liang WANG
Author Information
1. China Animal Health and Epidemiology Center, Qingdao 266032, China.
- Publication Type:Journal Article
- MeSH:
Amantadine;
therapeutic use;
Animals;
Antiviral Agents;
therapeutic use;
Chickens;
Drug Resistance, Viral;
genetics;
Influenza A virus;
drug effects;
genetics;
Influenza in Birds;
drug therapy;
virology;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Chinese Journal of Virology
2010;26(5):392-395
- CountryChina
- Language:Chinese
-
Abstract:
Mutation in any of five key amino acid residues (at positions 26, 27, 30, 31 and 34) within the M2 protein of influenza A viruses leads to resistance against the amantodine class of anti-influenza drugs. In this study, a pyrosequencing method was described to rapidly detect established five molecular markers of resistance to M2 blockers, amantadine. The residues L26, V27, A30, S31 and G34 in the M2 protein were targeted for pyrosequencing, and 94 avian influenza viruses were used to perform the amantadine resistance analysis. Our results showed that most of avian influenza viruses were amantadine resistant, Mutations V27I and S31N were founded in these isolates.
