Etiology of hand-foot-and-mouth disease and genetic characteristics of Coxsackievirus A16 in Beijing, 2009.
- Author:
Mei QU
1
;
Jie LI
;
Lei JIA
;
Xiao-Juan TAN
;
Zhi-Yong GAO
;
Han-Qiu YAN
;
Jing GUO
;
Xi-Tai LI
;
Xin-Yu LI
;
Quan-Yi WANG
;
Wen-Bo XU
;
Fang HUANG
Author Information
1. Department of Infectious and Endemic Diseases Control, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
- Publication Type:Journal Article
- MeSH:
Child;
Child, Preschool;
China;
epidemiology;
Enterovirus A, Human;
classification;
genetics;
isolation & purification;
Female;
Hand, Foot and Mouth Disease;
epidemiology;
virology;
Humans;
Infant;
Male;
Molecular Sequence Data;
Phylogeny
- From:
Chinese Journal of Virology
2010;26(6):432-436
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to reveal the pathogen spectrum of hand-foot-and-mouth disease (HFMD) and genetic characteristics of Coxsackievirus A16 (CoxA16) isolates in Beijing in 2009. From 1044 clinical specimens collected from 975 HFMD cases at Beijing Pediatrics Hospital, Beijing You'an Hospital and Beijing Ditan Hospital in 2009, viral nucleic acids of enterovirus were detected by reverse transcriptase polymerase chain reaction (RT-PCR). Enterovirus isolations were conducted with rhabdomyosarcoma (RD) cell line on 200 throat swabs having positive RT-PCR results. Sequencing and analyses of VP1 encoding gene were performed on 9 CoxA16 isolates in this study. The results showed that CoxA16 (49.4%) and EV71 (36.4%) were the major pathogens for the epidemics of HFMD in 2009 in Beijing, and CoxA16 was the predominant serotype, while there were also other enterovirus co-circulating, such as CoxA4, CoxA10, and CoxA9; the CoxA16 strains prevalent in Beijing in 2009 belonged to subgenotype B1a and B1b.
