Sequence analysis of the phosphoprotein gene of peste des petits ruminants virus of Chinese origin.
- Author:
Jing-yue BAO
1
;
Wen-ji ZHAO
;
Lin LI
;
Zhi-liang WANG
;
Guo-zhen WU
;
Xiao-dong WU
;
Chun-ju LIU
;
Qing-hua WANG
;
Jun-wei WANG
;
Yu-tian LIU
;
Jin-ming LI
;
Ying-li WANG
Author Information
1. National Diagnostic Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao 266032, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
China;
Female;
Goat Diseases;
virology;
Goats;
Molecular Sequence Data;
Peste-des-Petits-Ruminants;
veterinary;
virology;
Peste-des-petits-ruminants virus;
chemistry;
genetics;
isolation & purification;
metabolism;
Phosphoproteins;
chemistry;
genetics;
metabolism;
Sequence Analysis;
Sequence Homology, Amino Acid;
Viral Proteins;
chemistry;
genetics;
metabolism
- From:
Chinese Journal of Virology
2011;27(1):26-33
- CountryChina
- Language:Chinese
-
Abstract:
The nucleotide sequences of P gene from a field strain of peste des petits ruminants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The P gene is 1,655 nucleotides long with two overlapping open reading frames (ORFs). The first ORF is 1530 nucleotides long and would produce P protein of 509 amino acid residues. The second ORF is 534 nucleotides long and would produce C protein of 177 amino acid residues. The first ORF produces a second mRNA transcript of 897 nucleotides long with an extra G nucleotide at position 751. Translation from this mRNA would produce V protein of 298 amino acid residues. The nucleotide and deduced amino acid sequence were compared with the homologous region of other PPRV isolates. At the amino acid level, the "China/Tib/Gej/07-30" shares homology of 86.10%-97.3%, 84.3%-94.9%, and 82.9%-96.3% for P, C, and V proteins respectively. Several sequence motifs in the P genes were identified on the basis of conservation in the PPRVs and the morbilliviruses.
