Peptide mapping of H-2d restricted T-cell epitope against six antigens of HIV-1 subtype B'/C by ELISPOT assay.
- Author:
Xiang-rong QI
1
;
Ying-ying GAO
;
Rou-jian LU
;
Yao DENG
;
Xin MENG
;
Wen-jie TAN
;
Li RUAN
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Enzyme-Linked Immunospot Assay;
methods;
Epitopes, T-Lymphocyte;
chemistry;
genetics;
immunology;
Female;
H-2 Antigens;
chemistry;
genetics;
immunology;
HIV Antigens;
chemistry;
genetics;
immunology;
HIV Infections;
immunology;
virology;
HIV-1;
classification;
genetics;
immunology;
Histocompatibility Antigen H-2D;
Humans;
Mice;
Mice, Inbred BALB C;
Molecular Sequence Data;
Peptide Mapping;
methods
- From:
Chinese Journal of Virology
2011;27(1):34-43
- CountryChina
- Language:Chinese
-
Abstract:
The purpose is to screen and identify the specific H-2d restricted T-cell epitopes. These epitopes are used to investigate the cellular immune response of BALB/c (H-2d) mice immunized with a HIV-1 vaccine which expresses six antigens of gp160, gag, pol, rev, tat and nef of HIV subtype B'/C. A replicating DNA vaccine and a non-replicating recombinant vaccinia virus vector, both expressing the six antigens mentioned above, were used to immune BALB/c (H-2d) mice in a prime-boost regiment. The six peptide libraries of HIV B'/C corresponding respectively to the six complete antigens were pooled according to a designed matrix format and used to test for IFN-gamma production from splenocytes of immunized mice by an enzyme-linked immunospot (IFN-gamma ELISPOT) assay. The ELISPOT data indicated that two of seven Gag-specific T-cell epitope peptides were identified to be the novel epitopes. One of three Pol-specific T-cell epitope is unreported. One novel epitope was confirmed in two gp160-specific T-cell epitope peptides. One Nef-specific T-cell epitope was identified. Three Tat-specific T-cell epitope peptides were continuous sequences in Tat peptide library and all contained either complete or partial sequence reported. Rev-specific T-cell epitope was not be found. The specific T-cell epitopes (H-2d restricted) were identified by IFN-7 ELISPOT assay, which could be used to detect the cellular immune response of BALB/c mice immunized with the HIV-1 vaccine expressing six antigens of gp160, gag, pol, rev, tat and nef of HIV subtype B'/C.
