Biological and phylogenetic analysis of first isolate of Tahyna virus in China.
- Author:
Zhi LU
1
;
Shi-hong FU
;
Xin-jun LU
;
Song ZHANG
;
Su-xiang TONG
;
Chun-da GAO
;
Xin-hua YAO
;
Guo-dong LIANG
Author Information
1. State Key Laboratory for Infectious Disease Control and Prevention, Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. Liflit@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
China;
Encephalitis Virus, California;
genetics;
isolation & purification;
physiology;
Evolution, Molecular;
Female;
Fluorescent Antibody Technique;
Mice;
Nervous System;
virology;
Phylogeny;
Polymerase Chain Reaction;
Sequence Analysis, DNA
- From:
Chinese Journal of Virology
2011;27(2):97-102
- CountryChina
- Language:Chinese
-
Abstract:
In 2006, the first Chinese Tahyna virus isolate (XJ0625) was obtained in Xinjiang province and human infection were found in the same region. In this study, cell culture, animal experiments, electron microscopy, immunofluorescence assay and cross neutralization tests were performed to see the cell susceptibility, animal pathogenicity, morphology and antigenic and other biological characteristics of XJ0625. In addition, molecular biology software was used to analyze the characteristics of molecular evolution. The results showed that BHK-21 cell line was susceptible to XJ0625 and the virus was lethal to suckling mice when injected by intracranial ways. Similar to the other Bunyavirus, Tahyna virus is spherical enveloped virus under electron microscopy. XJ0625 infected cells showed strong fluorescent signal and could be neutralized by immune asities fluid with immnity to protype Tahyna virus Bardos 92. The sequence of the S and M segments showed 91.8% and 81.9% homology with Bardos 92.
