The effects of a hot water soluble extract (S-03) isolated from Isatis indigotica root on influenza A and B viruses in vitro.
- Author:
Zi-Feng YANG
1
;
Yu-Tao WANG
;
Sheng QIN
;
Sui-Shan ZHAO
;
Yun-Shi ZHAO
;
Qin LIN
;
Wen-Da GUAN
;
Qun-Di HUANG
;
Zi-Yao MO
;
Chu-Yuan LI
;
Nan-Shan ZHONG
Author Information
1. Clinical Virology Division, State Key Laboratory of Respiratory Diseases, Guangzhou Medical University, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China. jeffyah@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Dogs;
Fluorescent Antibody Technique;
Hemagglutination Inhibition Tests;
Influenza A virus;
drug effects;
Influenza B virus;
drug effects;
Isatis;
chemistry;
Plant Extracts;
pharmacology;
Plant Roots
- From:
Chinese Journal of Virology
2011;27(3):218-223
- CountryChina
- Language:Chinese
-
Abstract:
This study was to investigate the antiviral effects of a hot water soluble extract S-03 isolated from Isatis indigotica root on different subtypes of influenza A and B viruses in MDCK cell cultures, using plaque reduction, immunofluorescence and hemo-agglutination inhibition (HAD) assays. Chemical analysis of the extract S-03 showed that it contained high proportion of polysaccharides. The antiviral effects in vitro showed that the S-03 had no effect on different influenza viruses if the drug was used before virus adsorption, but S-03 showed obvious activities against influenza viruses if treatment after virus adsorption or direct reaction of drug and virus before virus adsorption. Hemagglutination inhibition assay showed that S-03 inhibited HA activities of different human influenza viruses (inhibition concentration ranged from 3.12 to 25 mg/mL), avain influenza viruses (inhibition concentration ranged from 25 to 50 mg/mL). The antiviral effects of S-03 on different influenza A and B viruses in vitro might be through the inhibition of the HA to prevent infection.
