A novel method for multiplex detection of gastroenteritis-associated viruses.
- Author:
Yan LIU
1
;
Zi-Qian XU
;
Jin-Song LI
;
Miao JIN
;
Wei-Xia CHENG
;
Xun GONG
;
Hui-Ying LI
;
Wan-Zhu YANG
;
Meng-Jie YANG
;
Xiu-Mei HU
;
Xue-Jun MA
;
Zhao-Jun DUAN
Author Information
1. State Key Laboratory of Molecular Virology and Genetic Engineering, Institute of Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
- Publication Type:Journal Article
- MeSH:
Gastroenteritis;
virology;
Humans;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
Sensitivity and Specificity;
Viruses;
isolation & purification
- From:
Chinese Journal of Virology
2011;27(3):288-293
- CountryChina
- Language:Chinese
-
Abstract:
To develop and optimize a simultaneous detection method of RotavirusA, Norovirus GI, GII, Sapovirus, human astrovirus, enteric adenoviruses and HBoV2 with GenomeLab GeXP analysis system. The sensitivity was verified to be 10(4) copies/microL with plasmids containing the viral targets in triplicate on different days, and no cross-reaction with enterovirus71, human Parechovirus and PicobirnavirusII was observed. Finally, we successfully developed a high throughout, rapid and maneuverable multiplex RT-PCR assay for simultaneous detection of seven viruses related with viral gastroenteritis, which provide a novel method for the molecular diagnosis of diarrhea-associated virus.
