Genetic characterization of poliovirus isolates from environmental sewage surveillance in Shandong, 2010.
- Author:
Yan ZHANG
1
;
Sheng-Xiang JI
;
Xiao-Li ZHANG
;
Yan LI
;
Yong ZHANG
;
Ze-Xin TAO
;
Hai-Yan WANG
;
Shuang-Li ZHU
;
Li-Zhi SONG
;
Yi FENG
;
Yao LIU
;
Feng JI
;
Xiao-Juan LIN
;
Lei FENG
;
Yoshida HIROMU
;
Ai-Qiang XU
Author Information
1. Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China. zhangyan204@sina.com
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
China;
Humans;
Molecular Sequence Data;
Mutation;
Poliomyelitis;
prevention & control;
virology;
Poliovirus;
genetics;
isolation & purification;
Population Surveillance;
Sewage;
virology
- From:
Chinese Journal of Virology
2011;27(4):337-341
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the genetic characteristics of poliovirus isolates from environmental sewage surveillance in Shandong province, we collected sewage samples in Jinan and Linyi City. Serotyping and VP1/ 3D sequencing were performed on polioviruses isolated from the concentrated sewage samples, and VP1 mutation and recombination were analyzed. Thirty-two of sewage samples were collected, and polioviruses were detected in 10 of the samples with a positive rate of 31.3%. Eighteen Sabin strains were isolated including three type 1, nine type 2, and six type 3 polioviruses, and the number of nucleotide substitutions in VP1 coding region varied from 0 to 4. Recombination was found in three Sabin 2 and four Sabin 3 polioviruses. Analysis of neurovirulence sites of VP1 revealed that one Sabin 1 vaccine strain had a nucleotide change of A to G at nt 2749, one Sabin 2 strain had a nucleotide change of A to G at nt 2908, three Sabin 2 strains had a nucleotide change of U to C at nt 2909, and all six Sabin 3 strains had a nucleotide change of C to U at nt 2493. Poliovirus vaccine strains could be isolated from environmental sewage with a high rate of gene recombination and back mutation of neuvirulence-associated sites. None of wild-type poliovirus or vaccine-derived poliovirus was detected.
