Effect of osthole on bone metabolism in rat femoral tissues in vitro.
- Author:
Jian ZHOU
1
;
Bao-feng GE
;
Ping ZHEN
;
Xiao-ni MA
;
Li-juan YAN
;
Xiao-Yu GUO
;
Kui CHENG
;
Yu-Hai GAO
;
Wen-Gui SHI
;
Ke-ming CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Bone Morphogenetic Protein 2; metabolism; Calcium; metabolism; Core Binding Factor Alpha 1 Subunit; metabolism; Coumarins; pharmacology; Femur; drug effects; metabolism; In Vitro Techniques; Male; Osteoprotegerin; metabolism; RANK Ligand; metabolism; Rats; Rats, Sprague-Dawley
- From: Acta Academiae Medicinae Sinicae 2013;35(5):561-566
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of osthole on bone metabolism in rat femoral tissues in vitro.
METHODSThe rat femoral tissues were isolated in vitro. The optimal concentrations of ostehole (1×10(-5) mol/L) and estradiol (1×10(-8) mol/L) (the positive control) were selected by alkaline phosphatase activity (ALP). The ALP and calcium levels were detected by commmerical regents, and the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene 2, and bone morphogenetic protein-2 mRNA were determined by real-time reverse transcription-polymerase chain reaction.
RESULTThe osthole (1×10(-5) mol/L) significantly increased the activity of ALP, calcium level as well as the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene-2 and bone morphogenetic protein-2 mRNA in rat femoral tissues in vitro.
CONCLUSIONOsthole can improve calcium level and ALP activity and regulate the bone metabolism-related genes in rat femoral tissues.
