Expression of growth-associated protein 43 in the hippocampus of mesial temporal lobe epilepsy mouse model.
- Author:
Xiao-feng WU
1
;
Long-ze SHA
1
;
Zhi-qiang SHA
1
;
Yan SHEN
1
;
Qi XU
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dentate Gyrus; metabolism; Disease Models, Animal; Epilepsy; Epilepsy, Temporal Lobe; metabolism; GAP-43 Protein; metabolism; Hippocampus; metabolism; Kainic Acid; Mice; Seizures
- From: Acta Academiae Medicinae Sinicae 2013;35(6):589-594
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the temporal and spatial distribution of growth-associated protein 43(GAP-43)and phosphorylated growth-associated protein 43(p-GAP-43)in the dentate gyrus of mesial temporal lobe epilepsy(MTLE)mouse model.
METHODSMTLE mouse model was established by using the kainic acid(KA)induction. Immunohistochemistry and Western blotting were applied to detect the expressions of GAP-43 and p-GAP-43 in different stages of epileptogenesis.
RESULTSBoth in the epileptic and control mice, high GAP-43 expression level was detected in the dentate gyrus, hilus, and inner molecular layer of hippocampus. Decreased p-GAP-43 expression was detected 5 days, 2 weeks, and 5 weeks after KA-induced seizures.
CONCLUSIONThe decreased p-GAP-43 expression in the duration of seizure may play an important role in the synaptic reorganization of the sclerotic hippocampus.
