Knockdown of proteasome subunit α7 with small interfering RNA inhibits cell proliferation of K562 cell line.
- Author:
Tao QIN
1
;
Cui-qing FAN
1
;
Ning ZHU
2
;
Yan SHEN
1
;
Mei-hong CHEN
1
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; Cell Line; Cell Proliferation; genetics; Down-Regulation; Gene Knockdown Techniques; Humans; K562 Cells; Proteasome Endopeptidase Complex; genetics; metabolism; RNA, Messenger; RNA, Small Interfering; therapeutic use; Transfection
- From: Acta Academiae Medicinae Sinicae 2013;35(6):601-606
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of human proteasome subunit Α7(PSMA7)gene silencing by small interfering RNA(siRNA)on human myeloid leukemia cell line K562.
METHODSPSMA7 gene-specific siRNA was chemically synthesized and transfected into K562 cell line by HiPerFect. The expression level of PSMA7 protein was detected by Western blot analysis. Cell proliferation was determined by MTS and cell counting. Cell cycle distribution was measured by flow cytometry. The expressions of Cyclin A, D, and E were detected by Western blot analysis. The apoptotic ratio was determined by flow cytometry.
RESULTSPSMA7 protein was evidently silenced 48 hours after transfection of the PSMA7-specific siRNA into K562 cell line. The proliferation of the cells was markedly inhibited, and the percentage of S phase cells decreased. However, no apoptosis was observed. The expressions of Cyclin A and E were down-regulated.
CONCLUSIONKnockdown of PSMA7 down-regulates the expression of Cyclin A and E and thus decreases the proportion of cells in S phase as a result, the proliferation of K562 cell line is inhibited.
