Identification of protein peroxiredoxin 2 related to crystalline NiS-induced neoplastic transformation.
- Author:
Wei-dong JI
1
;
Jia-kun CHEN
;
Jia-chun LÜ
;
Zhong-liang WU
;
Su-mei FENG
;
Fei YI
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; Cell Line; Cell Transformation, Neoplastic; chemically induced; metabolism; Epithelial Cells; cytology; drug effects; metabolism; Humans; Nickel; toxicity; Peroxiredoxins; metabolism; Proteome
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(4):267-270
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide evidence for illustrating the molecular mechanism of nickel carcinogenesis, and to identify the differential expression of protein in crystalline NiS-induced neoplastic transformation of human bronchial epithelial cell by proteomics technology.
METHODSTwo dimensional electrophoresis (2-DE) and the ImageMaster 3.10 software were used to analyze the differential expression of protein, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) combined with database search was applied to identify protein peroxiredoxin 2 (PDX2) related to malignant transformation.
RESULTSThe good 2-DE pattern including resolution and reproducibility was obtained. Nearly 700 expressed proteins per 2-D gel were isolated with molecular weights (MW) ranging from 14,400 to 94,000 KD and pI 3 - 10. A protein PDX2 with MW 21,890 KD, pI 5.66, which was highly expressed in malignantly transformed cell, was identified using MALDI-TOF-MS.
CONCLUSIONPDX2 was involved in malignant transformation of human bronchial epithelial cell induced by crystalline nickel sulfide.
