Study on differential proteomic expression in human liver cells stimulated by trichloroethylene with proteomics.
- Author:
Jian-jun LIU
1
;
Hai-yan HUANG
;
Zhi-xiong ZHUANG
;
Xi-yi LI
;
Jian-hui YUAN
;
Fan YANG
;
Qin-zhi WEI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Dose-Response Relationship, Drug; Electrophoresis, Gel, Two-Dimensional; Hepatocytes; drug effects; metabolism; Humans; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trichloroethylene; toxicity
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(6):431-434
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the differential proteomic expression in human liver cells L-02 induced by different dosages of trichloroethylene (TCE).
METHODSHuman liver cells L-02 were treated with different concentrations of TCE and the solvent control (dimethylsulfoxide). The total cellular proteins were separated using 2DE and visualized with silver staining after TCE treatment. The images were analyzed with Image Master 2D Platinum 5.0 analysis software. The differentially expressed protein spots were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-TOF-MS).
RESULTSFifteen protein spots with significant difference were found, and went upward or downward or disappeared after the stimulation of TCE with different dosages, which indicated that TCE induced the change of the proteomic expression in the liver cells. The mass spectrum identification and the IPI human database retrieval were used for identifying 9 proteins related to the L-02 Liver cells induced by TCE.
CONCLUSIONThe result provides an insight to TCE-related molecular mechanism and which might be useful for further study of the TCE-associated proteins and molecular markers.
