Airborne fine particulate matter induced pulmonary inflammation as well as oxidative stress in neonate rats.
- Author:
Li-ren DING
1
;
Kai WANG
;
Baher FAHMY
;
Hua-hao SHEN
;
Stephania CORMIER
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Bronchoalveolar Lavage Fluid; chemistry; Glutathione; metabolism; Male; Oxidative Stress; Particulate Matter; toxicity; Pneumonia; etiology; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha; analysis
- From: Chinese Medical Journal 2010;123(20):2895-2900
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDAirborne fine particulate matter (PM) can induce pulmonary inflammation which may adversely affect human health, but very few reports about its effect on the neonate rats are available. This study aimed to observe the potential impact and toxicity of fine PMs on the airway in neonate rats.
METHODSPulmonary inflammation, cytotoxicity, histopathology, and antioxidants as well as oxidant products were assessed 24 hours after intratracheal instillation of fine PM consecutively for 3 days. Cytotoxicity of fine PM was measured in HEp-2 cells.
RESULTSRats treated with high dose fine PM developed significant pulmonary inflammation characterized by neutrophil and macrophage infiltration. The inflammatory process was related to elevated level of TNF-α and prooxidant/antioxidant imbalance in the lung. Cytotoxicity studies performed in human epithelial cells indicated that high dose fine PM significantly reduced cell viability.
CONCLUSIONThe study demonstrated acute exposure to fine PM induced airway inflammation as well as increased oxidative stress in addition to its direct toxic effect on airway epithelium cells.
