Proliferation of renal mesangial cells induced by very low density lipoprotein is mediated by p42/44 mitogen activated protein kinase.
- Author:
Guo-qing YU
1
;
Wei-jie YUAN
;
Ruo-lan CUI
;
Peng FU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Cycle; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Lipoproteins, VLDL; pharmacology; Male; Mesangial Cells; cytology; drug effects; Mitogen-Activated Protein Kinase 1; metabolism; Mitogen-Activated Protein Kinase 3; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Medical Journal 2010;123(19):2710-2713
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDThe plasma concentration of very low density lipoprotein (VLDL) is negatively correlated to renal function in glomerular diseases. Effects of VLDL on renal function have been partially attributed to the proliferation of mesangial cells. This study examined the potential role of the p42/44 mitogen activated protein kinase (MAPK) in mesangial cell proliferation induced by VLDL.
METHODSMesangial cells were treated with VLDL at different concentrations or for different time. The cell cycle of the mesangial cells was analyzed by XTT assay and flow-cytometry; MAPK activity was also assayed. In some experiments, cells were treated with VLDL together with or without 0.1 µmol/L PD 98059.
RESULTSTen to 500 µg/ml VLDL stimulated the proliferation of mesangial cells cultured in vitro in a concentration-dependent manner. The effect was associated with an increase in p42/44 MAPK activity. Increased proliferation of mesangial cells by VLDL was significantly attenuated by PD98059, a specific p42/44 MAPK inhibitor.
CONCLUSIONThese results indicate that the p42/44 MAPK pathway is an important regulator of mesangial cell proliferation and of renal functions.
