Extracts of danshen and chuanxinlian on human monocytic line THP-1 induced by TNF-alpha.
- Author:
Yujie LI
1
;
Qing YANG
;
Xiaogang WENG
;
Xiaoxing ZHU
;
Yiwei WANG
;
Xiaoni LIU
;
Xiao HAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Adhesion; drug effects; Cell Line; Cell Movement; drug effects; Drugs, Chinese Herbal; pharmacology; Humans; Lipid Metabolism; drug effects; Male; Monocytes; cytology; drug effects; metabolism; secretion; Rabbits; Salvia miltiorrhiza; chemistry; Tumor Necrosis Factor-alpha; pharmacology
- From: China Journal of Chinese Materia Medica 2010;35(8):1030-1033
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether extracts of Danshen and Chuanxinlian (SL) could promote the function recovery in pre-monocytic cell line THP-1 induced by TNF-alpha.
METHODSL extracts (0.125-2 g x L(-1)) were used to incubate THP-1 for 24 h before stimulation with TNF-alpha (20 microg x L(-1)), the adhesion, migration, lipid uptake and secretion of THP-1 were observed.
RESULTSL (0.5-2 g x L(-1)) had obvious effect on decreasing the THP-1 adhesion. The number of passed membrane was much fewer than that of control cells in SL (0.125-2 g x L(-1)). SL (0.125-2 g x L(-1)) reduced the total cholesterol content significantly. The levels of IL-6 in SL (2 g x L(-1)) were significantly decreased,and IL-10 was increased than that before the treatment.
CONCLUSIONSL extracts could promote the function recovery such as adhesion, migration, lipid uptake and secretion of THP-1 induced by TNF-alpha, which probably is one of the mechanisms of inhibit the inflammatory reaction in initiation and development of AS.
