Study on fingerprints correlated with pharmacodynamic of constituents in Herba Erigerontis against neurotoxicity induced by beta-amyloid peptide.
- Author:
Yong HUANG
1
;
Xiaolan QI
;
Zhizhong GUAN
;
Yonglin WANG
;
Aimin WANG
;
Cuibing LI
;
Mingyan CHI
Author Information
- Publication Type:Journal Article
- MeSH: Alzheimer Disease; drug therapy; Amyloid beta-Peptides; toxicity; Analysis of Variance; Animals; Cell Line, Tumor; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; pharmacology; therapeutic use; Erigeron; chemistry; Humans; Nervous System; drug effects; Neurotoxins; toxicity
- From: China Journal of Chinese Materia Medica 2010;35(8):1038-1041
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the neuroprotective effects of the constituents in Herba Erigerontis on neuroblastoma SH-SY5Y cells, and find out its possible material foundation in treating Alzheimer's disease (AD).
METHODDifferent combinations of the three constituents in Herba Erigerontis were prepared according to the orthogonality experiment, and the indexes (MTT reduction assay, lipid peroxidation and expressions of nAChR alpha7 protein)were observed upon the SH-SY5Y cells followed by treatment of these combinations and beta-amyloid peptide (AP). The pharmacology data thus obtained and peak data in UPLC fingerprint were analyzed through ANOVA and correlationship by SPSS to give the information of active possible material foundation.
RESULTConstituents B and C showed clear activity and peaks of 4, 7-12 did positive correlationship according to the correlation of fingerprints and pharmacology.
CONCLUSIONThis study makes a valid approach for deducing the active constituents even the exact compounds against neurotoxicity induced by Abeta by correlation of fingerprints and pharmacology.
